Anti-SFTPA1 Antibody

Product Name

Anti-SFTPA1 Antibody

View all Surfactant Protein A Antibodies

SKU/Catalog Number

PA1347

Size

100 μg/vial

Form

Liquid

Description

Boster Bio Anti-SFTPA1 Antibody catalog # PA1347. Tested in WB applications. This antibody reacts with Human, Rat.

Storage & Handling

At -20˚C for one year and at 4˚C for one month from date of receipt. Avoid repeated freezing and thawing.

Cite This Product

Anti-SFTPA1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1347)

Host

Rabbit

Contents

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human SFTPA1, different from the rat sequence by two amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1347 is reactive to Sftpa1 in Human, Rat

Applications

PA1347 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

26-35 kDa

Calculated molecular weight

26.157kDa

Background of Surfactant Protein A

Surfactant protein A is an innate immune system collectin. The gene encoding SP-A map to mouse chromosome 14.1. Surfactant protein-A, which plays a role in innate host defense in the lung, is also expressed in the Eustachian tube. We report that the frequency of specific surfactant protein-A haplotypes and genotypes differs between children with recurrent otitis media compared with a control population.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Rat

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of SFTPA1 using anti-SFTPA1 antibody (PA1347).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat lung tissue lysates,
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SFTPA1 antigen affinity purified polyclonal antibody (Catalog # PA1347) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SFTPA1 at approximately 26-35KD. The expected band size for SFTPA1 is at 26KD.

Gene/Protein Information For Sftpa1 (Source: Uniprot.org, NCBI)

Alternative Names

COLEC4; PSAP; PSPA; PSP-A; SFTP1; SFTPA1; SFTPA1B; SPA; SP-A; SPA1; SP-A1; surfactant protein A1B Sftpa1|S, SFT, SP-A, Sftp, Sftp-1, Sftp1|surfactant associated protein A1|pulmonary surfactant-associated protein A|PSAP|PSP-A|surfactant pulmonary associated protein A1

For more info on Sftpa1, check out the Sftpa1 Infographic

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In this infographic, you will see the following information for Sftpa1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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